Fig. 1
Effect of nilotinib (with or without telmisartan) and imatinib on adipocyte lipid accumulation and mRNA expression of Pparγ, Lpin1, Srebp1 and Glut4 in differentiating 3T3-F442A adipocytes. 1A Lipid droplets were stained with Oil Red O on day 10 following treatment with respective drugs/vehicle. Lipid bound Oil Red O was extracted with isopropyl alcohol and absorbance was measured at 450 nm. 1B Gene expression of Pparγ, Lpin1, Srebp1 and Glut4 were assessed by real-time PCR using Taqman assays-on-demand gene expression assays (Life Technologies) on a 7900HT Fast Real-Time PCR system (Life Technologies). Hprt was used as an endogenous control. The mRNA expression was calculated using the comparative Ct method according to the manufacturer’s protocol and the fold change for the gene of interest was expressed as 2^-(∆∆CT). Telmisartan was co-incubated with only one concentration of nilotinib (4 µM). Lopinavir (LPV), an anti-HIV drug, was used as a positive control. All experiments were repeated three times in triplicate. Statistical analyses were conducted by one-way ANOVA with Dunnett’s Test. Data represent Mean ± SD; p ≤ 0.05. *Vehicle vs NILO/LPV/IMA; †NILO4µM vs NILO4µM + TEL5µM. NILO: nilotinib, IMA: imatinib, TEL: telmisartan, LPV: lopinavir, Hprt: Hypoxanthinephosphoribosyltransferase
