Fig. 5
Ikaros binding induces activation of “poised” enhancers. a Heatmaps of H3K4me1 and H3K27Ac ChIP-seq signals at genome-wide activated enhancer regions (from previously “poised state”) at day 1, after Ikaros introduction, vs. day 0. Signals are centered on H3K4me1 peaks at day 1. b Increased expression of genes associated with activated enhancer regions at day 1 (red) vs. day 0 (black). c Motif analysis for activated enhancer regions. d Heatmaps of Ikaros, H3K4me1, and H3K27Ac ChIP-seq signals at activated enhancer regions (from the previously “poised” state at day 0) with Ikaros direct binding at day 0 vs. day 1 after induction of Ikaros. Signals are centered on Ikaros peaks at day 1. e Examples of activated enhancers (from the previously “poised” state at day 0) that are activated by Ikaros binding and associated genes
