Table 2 Mean size of PCR products after the 2nd PCR (containing the Illumina sequencing adaptors and barcodes)

From: Standardized next-generation sequencing of immunoglobulin and T-cell receptor gene recombinations for MRD marker identification in acute lymphoblastic leukaemia; a EuroClonality-NGS validation study

Gene

Amplicon length (bp)

TRB-VJ

309–407

TRB-DJ

300–408

TRG

256–360

TRD

309–450

IGH-VJ

484–681

IGH-DJ

266–358

IGK-VJ-Kde

296–384

intron-Kde

309–382