Fig. 2

Functional enrichment analysis of protein changes observed in AML vs. normal hemopoietic CD34⁺ cells using Metacore^TM. a Enrichment analyses using Process Networks (left panel) and enrichment by protein function (right panel) shows that ‘Transcription’ is the most significant Network. The Network establishes relationships between the genes from the dataset but does not cluster them according to a specific pathway. A false discovery rate (FDR) of 0.05 was applied. b Protein networks associated with the proteins up- or downregulated in the nuclei of AML patient blasts. The network was generated using direct interaction algorithm of MetaCore^TM (Clarivate Analytics). Nodes represent proteins with lines between nodes indicating protein interactions. Only connected nodes are shown. Arrow heads indicate the direction of the interaction. Node shapes represent the functional class of the proteins as shown in the graphic key (Supplementary Fig. S2b). Red and blue circles indicate up and down regulation respectively when compared with CD34⁺ nuclei. Interactome analysis using “Transcription Factor” algorithm identified CEBPA (p = 5.285e−08) and WT1 (p = 0.002825) as the most significant connected transcription factors in our protein dataset