Table 1 HKDC1-based peptides induce cell death in MNCs from ENKTL patients and SNK6 cells.

Peptide name	Peptide sequence	AA	IC₅₀ (µM)
Peptide name	Peptide sequence	AA	ENKTL	SNK6
Antp-HKC8	RQIKIWFQNRRMKWKK-LQQAQKEN	24	1.3 ± 0.4 (n = 3)	1.3 ± 0.3 (n = 4)
Antp-D-HKC8	RQIKIWFQNRRMKWKK-LQQAQKEN	24	1.0 ± 0.3 (n = 3)	1.2 ± 0.4 (n = 3)
PAS-HKC8	GKPILFF-LQQAQKEN	15	1.4 ± 0.4 (n = 4)	1.4 ± 0.3 (n = 3)
PAS-D-HKC8	GKPILFF-LQQAQKEN	15	1.1 ± 0.3 (n = 3)	1.3 ± 0.3 (n = 4)
TAT-HKC8	GYGRKKRRQRRRG-LQQAQKEN	21	1.9 ± 0.4 (n = 4)	2.0 ± 0.4 (n = 3)
TAT-D-HKC8	GYGRKKRRQRRRG-LQQAQKEN	21	1.5 ± 0.7 (n = 4)	1.7 ± 0.5 (n = 4)
Tf-HKC8	HAIYPRH-LQQAQKEN	15	0.5 ± 0.2 (n = 3)	0.7 ± 0.2 (n = 3)
Tf-Scram	HAIYPRH-EILNKEKK	15	>15.0 (n = 3)	>15.0 (n = 3)
Tf-D-HKC8	HAIYPRH-LQQAQKEN	15	0.2 ± 0.1 (n = 5)	0.3 ± 0.2 (n = 5)
Tf-D-Scram	HAIYPRH-EILNKEKK	15	>15.0 (n = 3)	>15.0 (n = 3)
Tf-HKC15	HAIYPRH-ITAVAKRLQQAQKEN	22	1.2 ± 0.4 (n = 3)	1.4 ± 0.3 (n = 3)
Tf-D-HKC15	HAIYPRH-ITAVAKRLQQAQKEN	22	0.9 ± 0.3 (n = 3)	1.2 ± 0.5 (n = 3)
Tf-D-Scram	HAIYPRH-NAAAKLAVEILNKEK	22	>15.0 (n = 3)	>15.0 (n = 3)
Tf-HKN15	HAIYPRH-MFAVHLMAFYFSKLK	22	>15.0 (n = 3)	>15.0 (n = 3)
Tf-D-HKN15	HAIYPRH-MFAVHLMAFYFSKLK	22	>15.0 (n = 3)	>15.0 (n = 3)

Cells were incubated with different concentrations of peptides (0.1–15 µM) for 6 h. The half-maximal cell death activity (IC50) value was determined by PI staining with subsequent FACS analysis. Results are expressed as mean ± SEM. The cell-penetrating sequence is underlined, the HKDC1-based targeting sequences are in italics, and the amino acids in the D-configuration are marked in bold.
AA amino acid numbers of peptides, Antp a 16 penetrating residue long sequence from the Drosophila antennapedia-homeodomain, D D-configuration, all the D-amino acids were synthesized as retro-inverso peptides, HKC8 last eight aa of the C-terminal in human HKDC1, HKC15 last 15 aa of the C-terminal in human HKDC1, HKN15 first 15 aa of the N-terminal in human HKDC1, IC50 half-maximal cell death activity value, PAS penetration-accelerating segment, Scram scramble peptide sequence, TAT HIV-1 TAT sequence, Tf transferrin receptor internalization sequence.

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