Fig. 2: Oligoclonality. | Leukemia

Fig. 2: Oligoclonality.

From: Immunoglobulin gene analysis in chronic lymphocytic leukemia in the era of next generation sequencing

Fig. 2

a GeneScan profiling of a CLL case showing a dominant clonal peak (385 bp) but also a very minor one (379 bp). Using Sanger sequencing, only the dominant clonal IGHV/IGHD/IGHJ gene rearrangement would be characterized. By contrast, NGS offers the possibility of providing sequence data for both rearrangements, as shown in these two types of visualization by Vidjil: either by “GeneScan-like” clonotype size (b), or by IGHV and IGHJ gene composition (c). The dominant clonotype (84.5% of all sequences) corresponds to a mutated (88.5% germline identity) IGHV4-4/IGHD1-26/IGHJ4 gene rearrangement, while the minor one (9.3% of all sequences) corresponds to a mutated (93.1% germline identity) IGHV3-7/IGHD3-16/IGHJ4 gene rearrangement. Both rearrangements are productive.

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