Fig. 4: Loss-of-function shRNA-screening identifies functional dependencies in CMML#1-MN1 cells. | Leukemia

Fig. 4: Loss-of-function shRNA-screening identifies functional dependencies in CMML#1-MN1 cells.

From: Effective drug treatment identified by in vivo screening in a transplantable patient-derived xenograft model of chronic myelomonocytic leukemia

Fig. 4

a Experimental outline of loss-of-function shRNA screening showing lentiviral transduction of CMML#1-MN1 cells with a shRNA pool, transplantation into NSGS recipient mice, cell harvest after 8 weeks and quantification of shRNAs by next-generation sequencing. b Representation of shRNAs in blood, bone marrow and spleen of NSGS recipient mice at 8 weeks after transplantation compared to baseline shRNA representation (baseline: transduced cells kept in vitro and quantified on day 3), (each column represents one mouse, n = 6).

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