Fig. 2: Loss of Men1 decreases the frequency of LIC.

A Serial replating of in vivo transformed Men1^f/f and Men1^wt MN1-driven AML transduced with Cre. Colony numbers (left panel) and cell number (right panel) per 500 cells plated in duplicates. Cumulative data from three independent experiments. Error bars represent mean ± SEM of biological replicates (n = 3 Men1^wt and n = 5 Men1^f/f MN1-driven AML). ***p < 0.0005, *p < 0.05, ns = non-significant, unpaired double-sided t-test. B Peripheral blood (PB) engraftment at D21 post-transplantation. Percentage of GFP⁺ in PB (Men1^wt + Cre: 1 × 10⁵ n = 4, 1 × 10⁴ n = 9, 1 × 10³ n = 12, 1 × 10² n = 12; Men1^f/f + Cre: 1 × 10⁵ n = 5, 1 × 10⁴ n = 9, 1 × 10³ n = 12, 1 × 10² n = 12). Cumulative data from two independent experiments, using three distinct Men1^f/f MN1-driven AML donors. Error bars represent mean ± SEM. *p < 0.05, **p < 0.005, unpaired double-sided t-test. C Survival curves (left) and determination the LIC frequency using ELDA (right) [36]. of recipients of 1 × 10⁵ (n = 4), 1 × 10⁴ (n = 9), 1 × 10³ (n = 12), 1 × 10² (n = 12) Men1^wt or Men1^f/f MN1-driven leukemic cells transduced with Cre. Cumulative data from two independent experiments. *p < 0.05, ****p < 0.0001, Log rank (Mantel–Cox) test.