Fig. 3: Men1^−/− MN1-driven leukemic cells exhaust in vivo.

A Serial replating of Men1^wt and Men1^−/− MN1-driven AML cells isolated from moribund recipients. Colony and cell numbers per 500 cells plated in duplicates. Cumulative data from two independent experiments. Error bars represent mean ± SEM of biological replicates (n = 5 Men1^wt and n = 6 Men1^−/−). *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.00005, unpaired double-sided t-test. B Peripheral blood (PB) engraftment at D21 post-transplantation. Percentage of GFP⁺ in PB in recipients of 50,000 Men1^wt and Men1^−/− MN1-driven AML cells (n = 15 animals for each condition over two independent experiments). Error bars represent mean ± SEM. ****p < 0.0001, unpaired double-sided t-test. C Survival of secondary recipients of 50,000 Men1^wt and Men1^−/− MN1-driven AML cells isolated from moribund recipients. n = 15 animals for each condition over two independent experiments. p < 0.0001, Log rank (Mantel–Cox) test. ** Signifies failure to properly re-arrange both allele (Men1^f/- leukemia).