Fig. 4: Loss of Men1 downregulates the MN1 leukemic program.

Men1^wt and Men1^−/− MN1-driven AML cells were isolated from moribund recipients and plated in methylcellulose for 7 days before RNA extraction. n = 3 biological replicates for each condition. A Volcano plot representing differentially expressed genes in in Men1^−/− versus Men1^wt MN1-driven AML cells. B GSEA showing downregulation of the MN1 leukemic program (Heuser et al. [16]) in Men1^−/− versus Men1^wt AML cells isolated from moribund recipients, after 7 days in methylcellulose. C GSEA showing enrichment of genes downregulated by loss of Kmt2a is MN1-driven leukemia (Riedel et al. [19]) in Men1^−/− versus Men1^wt MN1-driven leukemic cells isolated from moribund recipients, after 7 days in methylcellulose.