Table 3 Methods of MRD detection.

From: Measurable residual disease in chronic lymphocytic leukemia: expert review and consensus recommendations

Method

Sensitivity

Comment

References

4-color Flow

10−4

≥107 fresh leukocytes needed; assay useful in CD20 regimens has been reported [75]

Rawstron [25]

≥6-color Flow

10−5

≥2 × 106 fresh leukocytes needed

Rawstron [27, 28, 71]

8-color Flow

10−6

–

Letestu [76]

10-color Flow

10−5

–

Sartor [77]

ASO IGH RQ-PCR

10−5

Patient-specific primers needed

Böttcher [32]

ddPCRTM

10−5

Patient-specific primers needed; no CLL data reported

Drandi [27]

clonoSEQ® Assay

10−6

Multiplex polymerase chain reaction and next-generation sequencing [78]

Thompson [79]

  1. ASO IGH RQ-PCR allele-specific oligonucleotide immunoglobulin heavy locus polymerase chain reaction, CLL chronic lymphocytic leukemia, ddPCR droplet digital PCR, Flow flow cytometry.
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