Fig. 7: Depletion of Tregs unleashes CD8 and NK cell responses resulting in MM remission. | Leukemia

Fig. 7: Depletion of Tregs unleashes CD8 and NK cell responses resulting in MM remission.

From: Transient regulatory T-cell targeting triggers immune control of multiple myeloma and prevents disease progression

Fig. 7

A Experimental setup; Tregs were depleted in DEREG mice by administration of diphtheria toxin (DTx) at day 16/17 post-MOPC injection. In addition, starting with the Treg depletion, antibodies against CD4 (GK1.5 200 µg/week), CD8 (YTS169.4 initial injection: 400 µg, then 200 µg/week) or NK cells (anti-asialo-GM polyclonal, 35 µg/week) were given once weekly. B, C Disease progression was monitored with noninvasive BLI. B Representative BLI progression of one mouse/group. C Average radiance of n = 5–7 mice per group normalized to individual signals at day 16 is shown. Each line represents tumor signal of an individual mouse. Differences compared to DTx Treg depletion group: Kruskal–Wallis test with Dunn’s multiple comparisons and adjusted p values: *p ≤ 0.05, ** p ≤ 0.01, and ***p ≤ 0.001.

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