Fig. 6: POLR2M binds to E1, E2, and the TSS.

A Schematic representation of the PDE2A locus (chr11:72,605,000–72,644,500) with the indicated promoter (P, yellow) and enhancer (E1 or E2, blue) regions and transcriptional start sites (arrows). The first two exons of PDE2A are depicted with a black horizontal bar. The MIR139 encoding sequence is depicted in red. The location of the primer sets with the respective numbers used for the experiments in this figure is depicted in gray. B, C Fold enrichment of POL-II (yellow) and POLR2M (orange) on the depicted loci relative to IgG (white) as determined by ChIP-qPCR in Molm-13 cells are shown. Data are representative of three experiments. D Same as B and C, except that Molm-13 cells were treated with indicated concentrations of UNC1999 (different shades of orange). Data are representative of two independent experiments. E Schematic overview of the MIR139 locus. The primer sets to detect transcripts coming from E1 (green), E2 (red), and TSS (blue) are shown. The relative expression of transcripts in UNC1999-treated Molm-13-treated cells is shown below. The two-tailed unpaired student’s t test with Welch’s correction was used for statistical analysis in B–E. Data are representative of three experiments. All graphs are depicted as mean ± SEM.