Fig. 3: Stability of CFs for laboratories using GUSB as a reference gene. | Leukemia

Fig. 3: Stability of CFs for laboratories using GUSB as a reference gene.

From: Standardization of molecular monitoring of CML: results and recommendations from the European treatment and outcome study

Fig. 3

CFs were calculated and provided to laboratories on an annual basis. The stability of each CF was determined as either optimal (bright green), satisfactory (green) or unvalidated (amber) by comparison with the previous year’s value using the following criteria; Optimal (+/− 1.2 fold): Old CF/New CF = 0.83–1.2, Satisfactory (+/− 1.6 fold): Old CF/New CF = 0.63–1.58 or Unvalidated: Old CF/New CF < 0.63 or >1.58. The bars charts show the number of laboratories for each category, per year for GUSB reference gene data sets. Several laboratories submitted data for more than one reference gene or assay and therefore the number of data sets analysed may be greater than the number of participating laboratories.

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