Fig. 4: Inactivation of KDM4C results in loss of cell competition and reduced proliferation.
From: Histone demethylase KDM4C is a functional dependency in JAK2-mutated neoplasms
A Schematic depicting negative-selection competition assay. B Results of the negative-selection competition assay showing the relative percentage of RFP + sgRNA+ cells over time after transduction of JAK2-mutated HEL cells with indicated sgRNAs targeting luciferase control (sg-Luc), KDM4C (sg2-KDM4C; sg3-KDM4C) or RPA3 (positive control, sg-RPA3). n > 3. C Proliferation assayed by cell counting after trypan blue exclusion for Ba/F3-V617F-Cas9-Blast cells, SET-2-Cas9-Blast cells and HEL-Cas9-Blast cells transduced with sgRNAs targeting KDM4C, RPA3, or a non-targeting control (sgLuc). n > 3 independent experiments. D Schematic representation of the experimental procedure for CRISPR-Cas9 mediated knockout of KDM4C in human HEL cells followed by transplantation into xenograft mice at two different dilutions (30,000 cells; 60,000 cells). E Violin plot showing spleen weight of recipient animals at the timepoint of analysis (n = 9; two-tailed t-test). F Kaplan–Meier survival curves of humanized NXG recipient mice injected with two different dilutions of CRISPR-Cas9 edited human HEL cells.
