Fig. 4: Detection of tumor growth, engraftment and glucose consumption of CD147-KD KiJK- and SUDHL-1 lymphoma cells in mouse xenotransplant models.
From: CD147 a direct target of miR-146a supports energy metabolism and promotes tumor growth in ALK+ ALCL
A Exemplary PET/MR-scans of the tumor growth dynamic 3 and 4 weeks after implantation of CD147-KD (pF.CD147) KiJK and SUDHL-1 lymphoma cells (red arrows indicate tumors). B MRI-derived tumor volume 3-, and 4-weeks post tumor cell implantation (mean ± SD; CD147-KD: n = 5; untreated: n = 5, each symbol represents a mouse tumor measurement). CD147-KD precludes growth in KiJK and SUDHL-1 lymphoma cells. Two controls are included at week 4 because 3 of 5 mice died after week 3. For statistical analysis Wilcoxon rank-sum test was used, *p < 0.05. C MRI-derived tumor volume growth until day 29 post tumor cell implantation (mean ± SD; CD147-KD: n = 5; untreated: n = 5, SD is depicted by non-continuous lines). CD147-KD resulted in reduced tumor growth in KiJK and SUDHL-1 cells. Statistical analysis was performed using unpaired t-test (for only two group comparison) and repeated measures ANOVA: Significant differences at different time points and ending point are indicated as **P < 0.001 ****P < 0.0001. D The engraftment (%) of KiJK- and SUDHL-1-lymphomas was reduced after pF-CD147-treatment (KiJK: CD147-KD n = 5, untreated n = 5; SUDHL-1: CD147-KD n = 5, untreated n = 5). E [18F]FDG-uptake was reduced after CD147-KD in KiJK and SUDHL-cells (mean ± SD; CD147-KD: n = 5; untreated: n = 5, each symbol represents a mouse tumor measurement). For statistical analysis Wilcoxon rank-sum test was used, *p < 0.05. F Macroscopic images of representative tumors derived from the mice described in A and Hematoxylin and Eosin (H&E) staining of representative tumors of CD147-KD and untreated KiJK cells (original magnification 12.5×). G Graph plots of CD31 and VEFGR2 immunohistochemistry quantification of control tumors (n = 3) and tumors with CD147-KD cells (KiJK; n = 3). For statistical analysis Unpaired t-test ****p < 0.0001). H Comparative immunohistochemical analysis of representative tumors of CD147-KD and untreated KiJK cells stained with ALK, CD30, p-STAT3, CD147, MMP7, MCT1, (Immunohistochemistry, original magnification 400×), CD31 and VEGFR2 (Immunohistochemistry, original magnification 200×).
