Fig. 5: Impairment of CD147-KD and CD147-KO results in mitochondrial damage and accumulation of depolarized mitochondria in ALK+ ALCL cell lines.
From: CD147 a direct target of miR-146a supports energy metabolism and promotes tumor growth in ALK+ ALCL
A According to the ratio of MDR to MG staining using FACS analysis, two populations were recognized MDR/MG high and MDR/MG low. Both populations were determined in SUDHL-1 control (CD147 WT n = 2), SUDHL-1 cells with CD147-KO (n = 4) and in SUDHL-1 control with hypoxia. B Bar blots showing the percentage of cells with MDR/MG low staining (left) and the MDR/MG ratio (right) displayed among the groups. A high percentage of low staining cells and reduced MDR/MG ratio, correlates to low mitochondrial activity and membrane potential. Each point represents the average of three biological replicate measurements. Unpaired t-test, **p < 0.01, ***p < 0.001. C Electron microscopy demonstrates reduced number of mitochondria (M), many swollen with reduction of the number of cristae and cristae-length in ALCL cell lines (SUDHL-1 and KiJK) CD147 KD cells when compared to intact mitochondrial morphology of control cells. Additionally, note the accumulation of lipid vesicles (L) in the cytoplasm of ALK+ ALCL CD147 KD cells. D Bar plot displaying the quantification of Crista number per mitochondrion in CD147 WT and CD147KD cells. All mitochondria per cell were counted, (WT, n = 10 and KD n = 10 per every cell line). Unpaired t-test, **p < 0.01, ***p < 0.001.
