Fig. 3: Functional validation of GC-responsive sites using high-throughput reporter assays. | Leukemia

Fig. 3: Functional validation of GC-responsive sites using high-throughput reporter assays.

From: Epigenomic profiling of glucocorticoid responses identifies cis-regulatory disruptions impacting steroid resistance in childhood acute lymphoblastic leukemia

Fig. 3

A A schematic of diagram of ATAC-STARR-seq is provided. B IGV browser read count tracks of 697 ATAC-seq and H3K27ac, as well as read counts per million tracks of ATAC-STARR-seq DNA input and RNA output in 697 cells at 0, 6, and 24 h are provided. Examples of two active STARR-seq sites in 697 cells near the RCSD1 GC-responsive gene are denoted by red arrows. C Number of active STARR-seq sites identified after 0 h or after 6 + 24 h (GC) of prednisolone treatment in 697 and Nalm6 cells is provided at the left. Percentage of GC-active STARR-seq sites in 697 (top) and Nalm6 (bottom) cells that map to GC-responsive ATAC-seq sites exhibiting enhanced (blue and red) or reduced (light blue and pink) open chromatin accessibility following GC treatment is provided at the right.

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