Fig. 4: Impact of intragenic variant rs7045812 on GC-responsiveness and TLE1 expression. | Leukemia

Fig. 4: Impact of intragenic variant rs7045812 on GC-responsiveness and TLE1 expression.

From: Epigenomic profiling of glucocorticoid responses identifies cis-regulatory disruptions impacting steroid resistance in childhood acute lymphoblastic leukemia

Fig. 4

A IGV browser tracks of Nalm6 GR, ATAC-seq and H3K27ac at HGR spanning variant rs7045812 after 2 h of GC treatment is shown. The genome sequence spanning variant rs7045812 (highlighted in red) is shown and the GRE sequence (negative strand; arrow) is underlined. The GRE motif (positive strand) is shown below and the location of rs7045812 is denoted. B Luciferase reporter assay testing a 300-bp fragment of DNA centered on the reference C allele and alternative T allele in the presence or absence of prednisolone (5 µM; n = 5 per group). C GC drug viability results displaying the percentage of viable cells after 72 h of prednisolone treatment in parental/wild-type (WT) and TLE1 HGR deleted (Del) Nalm6 cells (250 nM; n = 48 per group).

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