Fig. 1: MSI2 expression is upregulated in SOX11+ compared to SOX11– MCL cases and associated with poor survival in MCL patients.

A Enrichment plots obtained by GSEA using GEP microarray data from 30 SOX11+ and 24 SOX11– MCL primary tumors (GSE79196), showing significant enrichment of HSC, LSC and NOS-related target gene sets in SOX11⁺. Normalized enrichment score (NES), P value (P val) and false discovery rate (FDR) are shown. Statistical significance is considered when FDR < 0.2. B Venn diagram illustrating the overlap between differential expressed genes (DEG) between SOX11+ and SOX11– MCL primary cases (GSE79196) (yellow circle, 816 genes with adjusted P value <0.05 and absolute log₂ fold change >0.7; see Supplementary Table S6), genes whose gene ontology biological process definition is related to stem cells (GO stem cell-related genes) (red circle, 373 genes; see Supplementary Table S7) and SOX11-bound genes (GSE35021) (orange circle, 1909 genes found by SOX11-specific ChIP-chip in MCL cell lines). C mRNA expression levels of PROX1, PRDM15, MSI2 and SOX5 genes in 30 SOX11+ and 24 SOX11– MCL primary cases (GSE79196). Expression levels were calculated by average of all the microarray probes of each gene, except for MSI2 (see methods). The significance of difference was determined by unpaired two-tailed Student’s t-test (Welch’s correction was used for SOX5): **P value <0.01, ***P value <0.001. D Kaplan–Meier curve showing the association of MSI2 mRNA expression and OS in 40 MCL primary cases (GSE79196) (Supplementary Table S1). MSI2 high and low values were defined by maximally selected rank statistics (cutoff = 8.46 expression units). The P value of log-rank test (P), the risk table (no. at risk), the hazard ratio (HR) with 95% confidence interval (CI) and Cox regression P value (P) are shown.