Fig. 3: Single-cell RNA sequencing reveals increases in numbers and changes in cellular gene expression of NFIC overexpressing monocytes.
a Graphical representation of single-cell mRNA sequencing of NFIC overexpressing normal blood cells. 8000 GFP+ sorted cells were processed for nuclei isolation, library preparation, and single-cell RNA sequencing using Chromium 10x genomics. b t-distributed stochastic neighbor (t-SNE) embedding analysis for representing different cell clusters identified based on principal component analysis (PCA). Cells are colored on the basis of different cell type clusters formed. c Bar graph represents total cell numbers within each indicated cell type clusters in GFP only (Control) vs NFIC overexpressing (NFIC) group. d Differential gene expression analysis between NFIC overexpressing monocytes vs control identified top canonical pathways and e disease and molecular function terms enriched after differential gene expression within the monocytes cell cluster using IPA. Each bar graph represents the cellular pathway and x-axis represents the −log10(P) values which determine the level of significance and numbers besides each bar shows number of genes altered in within that pathway. f Network map for all significant genes associated within cell survival pathway with three most enriched functional nodes and their interconnections. Red shades indicate upregulation of genes whereas green shades indicate downregulation. A complete list of these genes is given in Supplementary Table 3. The network map was generated using IPA Path Designer tool. Legends show in the text box at the right of the map.
