Fig. 5: NFIC knockdown reduces survival of AML cells by inducing apoptosis and cell cycle arrest. | Leukemia

Fig. 5: NFIC knockdown reduces survival of AML cells by inducing apoptosis and cell cycle arrest.

From: Nuclear factor I-C overexpression promotes monocytic development and cell survival in acute myeloid leukemia

Fig. 5: NFIC knockdown reduces survival of AML cells by inducing apoptosis and cell cycle arrest.

a Cell cycle analysis of NFIC KD AML cell lines. Bar graphs represent percentage of cells in G2/M cell cycle phase. Cell cycle analysis was performed after four days of infection with NFIC shRNAs as compared to scrambled RNA. Data represents mean±1 SD with *p < 0.05 and **p < 0.01 as analyzed by Dunnett’s multiple comparison test. b Bar graph represents percentage of Annexin-V+ cells. AML cell lines were infected with NFIC shRNA (sh488 and sh494) or scrambled shRNA (shScr) and after four days, stained with Annexin-V and 7-AAD and analyzed by flow cytometry. The percentage of Annexin-V+ (early apoptotic) and Annexin-V+/7-AAD+ (late apoptotic) were pooled together to represent the extent of apoptosis in these cells. Data is represented as mean±1 SD with *p < 0.05 and **p < 0.01 as analyzed by Dunnett’s multiple comparisons test. c Bar graph represents percentage of Annexin-V+ cells. Primary AML patient PBMCs were infected with NFIC shRNAs or scrambled control. After four days cells were analyzed by flow cytometry. Bar graphs represent mean±1SD with *p < 0.05 as analyzed by Dunnett’s multiple comparison test, d Western blots representing expression of Apoptosis-inducing factor (AIF) Mol. Wt. 66 KDa. Infection efficiency was >95% in all cases. GAPDH was used for endogenous loading control.

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