Fig. 5: Genomic and transcriptomic analysis of the FLT3 locus. | Leukemia

Fig. 5: Genomic and transcriptomic analysis of the FLT3 locus.

From: Cre recombinase expression cooperates with homozygous FLT3 internal tandem duplication knockin mouse model to induce acute myeloid leukemia

Fig. 5

A Visualisation of whole genome sequencing (WGS) reads over the endogenous Flt3 intron between exon 15 and 16 (top) and the neomycin (Neo) resistance cassette with flanking Flt3 intronic sequence (bottom) in Cre+FLT3ITD/ITD pre Cre expressing cells demonstrating retained targeting vector reads that map to a Neo-resistance cassette. This is not present in WT cells or after Cre expression. B RNA-sequencing reads over the endogenous Flt3 intron between exon 15 and 16 (top) and the Neo-resistance cassette with flanking Flt3 intronic sequence (bottom) consistently showing reads in FLT3ITD/ITD cells but not in Scl-CreERT:FLT3ITD/ITD, Mx1-Cre:FLT3ITD/ITD cells post Cre expression or Mx1-Cre:FLT3WT/WT.

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