Fig. 6: siRE-mod/LNPs cause long-term repression of RUNX1/ETO expression and function in vivo. | Leukemia

Fig. 6: siRE-mod/LNPs cause long-term repression of RUNX1/ETO expression and function in vivo.

From: Nanoparticle-mediated targeting of the fusion gene RUNX1/ETO in t(8;21)-positive acute myeloid leukaemia

Fig. 6

a Schematic illustration of RG mice transplantation and siRNA-LNPs treatment. Mice were injected with 3 mg/kg (i.p.) on day 1 and 2 followed by 1 mg/kg (i.v.) on day 3, 6, and 9, then humanly killed on day 12. Leukaemic cells were collected for downstream analysis. b Western blotting showing RUNX1/ETO, TERT, CCND2, GAPDH and CLTA expression in cells isolated from treated mice. *, two tumours from the same animal; all other lanes represent material from different animals. siRNA-LNPs-mediated RUNX1/ETO depletion in vivo led to induction of cellular senescence as shown by beta-galactosidase staining (c), inhibited proliferation ex vivo (d), and blocked colony formation capacity (e). Significance was tested by one-way ANOVA (c) or unpaired Student’s t tests (d, e).

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