Fig. 1: Thymocyte-expressed, positive selection-associated 1 (Tespa1) deficiency facilitates hematopoietic stem cell (HSC) expansion upon short-term stress.
From: Tespa1 facilitates hematopoietic and leukemic stem cell maintenance by restricting c-Myc degradation
A Quantitative real-time PCR (qPCR) analysis of Tespa1 mRNA expression in LSKs isolated from mice (left) at day 9 after 5-fluorouracil (5-FU) injection or (right) at day 13 after irradiation (IR) exposure (n = 3). Untreated mice were served as controls. LSK, Lin- Sca1+ c-Kit+. B, C The numbers (two femurs and tibias) of myeloid progenitors (MPs), LSKs, long-term HSCs (LT-HSCs), short-term HSCs (ST-HSCs), multipotent progenitors (MPPs) and signaling lymphocytic activation molecule (SLAM)-HSCs in the bone marrow (BM) of Tespa1+/+ and Tespa1-/- mice at day 9 following 5-FU injection (n = 5). MP, Lin- Sca1- c-Kit+; LT-HSC, Lin- Sca1+ c-Kit+ CD34- Flk2-; ST-HSC, Lin- Sca1+ c-Kit+ CD34+ Flk2-; MPP, Lin- Sca1+ c-Kit+ CD34+ Flk2+; SLAM-HSC, Lin- Sca1+ c-Kit+ CD150+ CD48-. *P < 0.05, **P < 0.01.
