Fig. 1: Reduced hydroxymethylation in TF-1 IDH2R140Q is reversed after AG-221 treatment. | Leukemia

Fig. 1: Reduced hydroxymethylation in TF-1 IDH2R140Q is reversed after AG-221 treatment.

From: Perturbed epigenetic transcriptional regulation in AML with IDH mutations causes increased susceptibility to NK cells

Fig. 1

A Heatmap showing z-scores of Δβ 5hmC values of the 2156 CpG sites which lose 5hmC in TF-1 IDH2R140Q mutant vs TF-1 IDH2WT cells in steady-state and AG-221 treatment (4 and 7 days). B Genomic location in 450 K enhancers, promoters or gene bodies (based on UCSC RefGene group; not annotated, 3’UTR and 5’UTR are not shown) of the hyperhydroxymethylated CpG probes (Δβ 5hmC > 0.05) at 4 and 7 days of AG-221 treatment in TF-1 IDH2R140Q mutant cells, comparing to location of all CpG probes in the EPIC array. Enrichment was calculated using Fisher exact t-test. *p-value < 0.01. C Dotplots of 5hmC deltabeta value (Δβ-value 5hmC) of differentially hydroxymethylated CpG probes (absolute(Δβ-value 5hmC) > 0.05) in relation to their 5mC deltabeta value (Δβ-value 5mC) between TF-1 IDH2R140Q cells treated with AG-221 for 4 days (Pearson correlation coefficient of −0.848) and 7 days (Pearson correlation coefficient of −0.815) and DMSO-treated control cells.

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