Fig. 2: Loss of MGA in vitro promotes cell growth.
From: Functional characterization of cooperating MGA mutations in RUNX1::RUNX1T1 acute myeloid leukemia
A Western blot analysis of MGA in WT and MGA-KO cells. B Flow cytometric analysis of WT (n = 3) and MGA-KO (n = 3) MOLM-13 cells treated with EdU for 2 h and stained with DAPI. C Cell growth assay of WT and MGA-KO MOLM-13 cells over time. Statistics: Two-way ANOVA with Šídák’s multiple comparisons test; error bars indicate the standard error of the mean from three or more biological replicates. D Volcano plot of differentially expressed genes (DEGs) from MGA-KO MOLM-13 cells. Red & Blue points represent upregulated and downregulated genes, respectively, and that have wild-type MGA binding at their promoter-TSS as determined by CUT&RUN. Green points represent nonsignificant transcriptional changes with MGA binding at promoter-TSS. E CUT&RUN coverage plots of MGA, PCGF6, and MYC occupancy on CCND2 and SMC1B in WT (blue) and MGA-KO (red) MOLM-13 cells. F Chromatin occupancy at CCND2 and SMC1B promoters from ATAC-Seq in WT (black) and MGA-KO (red) MOLM-13 cells. G Replicate tornado plots of ATAC-Seq showing the signal enrichment at the most stringent threshold - Up2 and Down2 (FC > 2, FDR < 0.05).
