Fig. 2: TRAF6 expression inversely correlates with mitochondrial function-related gene signatures in AML.
From: Metabolic reprogramming regulated by TRAF6 contributes to the leukemia progression
A Normalized enrichment scores (NES) from Gene Set Enrichment Analysis (GSEA) for the top 20 upregulated (red, top) and downregulated (blue, bottom), significantly altered gene sets in TRAF6low compared to TRAF6hi AML patient samples from the TCGA AML dataset [3]. Low and high TRAF6 expressions defined by: TRAF6low, Z score <1; TRAF6hi, Z score >1. B, C RNA sequencing analysis of two groups: (1) human leukemic cell lines with inducible shTRAF6, treated with or without doxycycline (DOX, 1 μg/mL) for 7 days, and (2) MLL-AF9;Traf6+/+ and MLL-AF9;Traf6−/− leukemic cells. The mitochondrial states were evaluated using GSEA profiles based on TRAF6low leukemic cells, with a focus on mitochondria-associated gene signatures organized by their P values (log10). Selected gene set enrichment plots of AML patients stratified based on low/high TRAF6 expression (D), HEL cells transduced with the inducible shTRAF6 (E), and MLL-AF9;Traf6+/+ and MLL-AF9;Traf6−/− leukemic cells (F). NES normalized enrichment score, DOX doxycyclin.
