Fig. 6: DAC induces immunogenic ERE MAPs. | Leukemia

Fig. 6: DAC induces immunogenic ERE MAPs.

From: Autophagy degrades immunogenic endogenous retroelements induced by 5-azacytidine in acute myeloid leukemia

Fig. 6

A Repartition of cancer-testis antigens (CTA) and ERE MAPs significantly altered by DAC in THP-1 cells. DE: differentially expressed, DACspecific: MAPs present in all three replicates of DAC and absent in CT. B Heatmap of average RNA expression of MAPs upregulated by DAC in normal tissues from GTEx (n = 12–50), normal hematopoietic cell populations (n = 3–16), or mTECs (n = 11). Boxes in which a MAP has an rphm > 8.55 are highlighted in black. C Comparison of immunogenicity scores (Repitope, left and BamQuery, right) among MAPs upregulated by DAC-treated THP-1 cells, MAPs from thymic stromal cells, AML tumor specific antigens (AML TSAs) and HIV MAPs. Thymic MAPs are negative control since they are not expected to be immunogenic, while AML TSAs and HIV MAPs are positive controls. Treatments refer to three days of daily 0.5 μM AZA or 30 nM DAC followed by 24-h discontinuation unless otherwise stated.

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