Fig. 3: In vitro CRISPR/Cas9-mediated screen to identify E2F members important for HSPC expansion. | Leukemia

Fig. 3: In vitro CRISPR/Cas9-mediated screen to identify E2F members important for HSPC expansion.

From: In vivo CRISPR/Cas9-mediated screen reveals a critical function of TFDP1 and E2F4 transcription factors in hematopoiesis

Fig. 3: In vitro CRISPR/Cas9-mediated screen to identify E2F members important for HSPC expansion.

A FACS analysis of the percentage of GFP+ (Cas9+) cells within the mCherry+ (sgRNA+) and mCherry- (sgRNA-) HSPCs on day two (top) and six (bottom) post transduction. B Heatmap of survival/proliferation scores in HSPCs treated with the indicated sgRNAs (n = 2, biological replicates). The color indicates decreased (orange) and increased (blue) survival/proliferation scores. C Western blot of TFDP1 and E2F4 three days post targeting with sgRNAs against Tfdp1 and E2f4. SgRosa26-1 was used as a negative control. Actin was used as a loading control. D Co-immunoprecipitation (Co-IP) using TFDP1 (top) and E2F4 as precipitating antibody (bottom) (n = 2, biological replicates).

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