Fig. 1: Gata2R396Q/+ mice display significant alterations in bone marrow hematopoiesis during adulthood.
From: Modeling GATA2 deficiency in mice: the R396Q mutation disrupts normal hematopoiesis
A Schematic representing CRISPR/Cas9 editing of Gata2 to produce Gata2R396Q/+ heterozygous mice. B Frequency of R396Q positive droplets in WT and Gata2R396Q/+ whole bone marrow (WBM) via digital droplet PCR (n = 4). C Complete blood count (CBC) data from young (10-12 weeks) and old (53-55 weeks) mice (n = 18). Absolute number of WBM cells D, mature cells E, maturing red blood cells (RBC) F, myeloid progenitors G, and common lymphoid progenitors (CLP) H in 8–10 week old mice (n = 7–11). I Proportion of CMPs, GMPs, or MEPs among myeloid progenitors in the BM of 8–10 week old mice (n = 7–11). J Absolute number of BM hematopoietic stem and progenitors (HSPCs) in 8–10 week old mice (n = 7–11). K Proportion of LT-HSC, ST-HSC, and MPP2 cells among BM LSK cells in 8–10 week old mice (n = 7–11). Representative flow plot L and quantification M of gMFI CD150 expression among BM HSPCs in 8–10 week old mice. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 WBC white blood cells, LY lymphocytes, NE neutrophils, MO monocytes, RBC red blood cells, PLT platelets, WBM whole bone marrow, LK lineage-c-Kit+; CMP common myeloid progenitor, GMP granulocyte-monocyte progenitor, MEP megakaryocyte-erythroid progenitor, CLP common lymphoid progenitor, LSK lineage-Sca-1+c-Kit+, LT-HSC long-term hematopoietic stem cell, ST-HSC short-term HSC, MPP2 multipotent progenitor 2, gMFI geometric mean fluorescence intensity.
