Fig. 3: The transcriptional signature of Gata2R396Q/+ hematopoietic progenitors reveals myeloid bias and a loss of HSC stemness.
From: Modeling GATA2 deficiency in mice: the R396Q mutation disrupts normal hematopoiesis
A UMAP projections of scRNA-seq from sorted WT or Gata2R396Q/+ BM LSK cells from 8–10 week old mice (n = 3-4 samples per condition). B UMAP projections of WT or Gata2R396Q/+ LSKs (blue) overlayed onto previously published scRNA-seq data from Kucinski et al. (gray), with indications of specific lineages. C Cluster annotations for LSK cells from WT and Gata2R396Q/+. D Proportions of specific cell types among WT or Gata2R396Q/+ LSKs (n = 3-4). E FeaturePlot displaying hscScore values among specific clusters in WT or Gata2R396Q/+ LSK cells. F Quantification and comparison of hscScores between specific clusters of WT or Gata2R396Q/+ LSKs. ns not significant, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Prog progenitor, Mono monocyte, DC dendritic cell, pDC plasmacytoid dendritic cell, Ery/Meg/Bas erythroid, megakaryocyte, basophil, Int intermediate, Lymp lymphoid, HSC hematopoietic stem cell.
