Fig. 4: Gata2R396Q/+ hematopoietic progenitors exhibit an accelerated aging phenotype.
From: Modeling GATA2 deficiency in mice: the R396Q mutation disrupts normal hematopoiesis
UMAP projections and GSEA analysis of HSC 1/HSC 2 A, Int Prog 1/ Lymp Prog B, and Int Prog 2/3/4 C clusters, showing terms negatively and positively enriched in Gata2R396Q/+ populations. D GSEA plots showing positive enrichment for Marrow HSC Aging in Gata2R396Q/+ cells from the indicated clusters. E Schematic for LPS in vivo treatment. F Frequency of LT-HSC cells normalized to PBS controls 5 days post in vivo LPS treatment of WT and Gata2R396Q/+ 8–10 week old mice (n = 4-5 per condition). Terms have an FDR < 0.05 and Gene % > 15%. ns not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. HSC hematopoietic stem cell, Lymp lymphoid, Prog progenitor, Int intermediate, LT-HSC long-term HSC, PBS phosphate buffered saline, LPS lipopolysaccharide.
