Fig. 6: Gata2R396Q/+ impairs fetal liver hematopoietic development and function.
From: Modeling GATA2 deficiency in mice: the R396Q mutation disrupts normal hematopoiesis
A Schematic of experiments utilizing WT and Gata2R396Q/+ E14.5 fetal liver (FL). B Absolute numbers of total FL, LSK, and hematopoietic stem and progenitor (HSPC) cells from WT and Gata2R396Q/+ littermates at E14.5 (n = 10-11 embryos). C Percentage of LT-HSC or MPP2 among LSK cells in E14.5 FL (n = 17–20 embryos). Representative flow plot D and normalized quantification E of gMFI CD150 among WT and Gata2R396Q/+FL HSPCs at E14.5 (n = 17–20 embryos). F Number of CFU subtypes from E14.5 FL in WT or Gata2R396Q/+ embryos (n = 10–12 embryos). G Schematic of experiments utilizing WT and Gata2R396Q/+ E12.5 FL. H Absolute numbers of total FL, LSK, and LT-HSC in E12.5 FL (n = 11–18 embryos). I Percentage of LT-HSC among LSK cells in E12.5 FL (n = 11–18 embryos). J Normalized quantification of gMFI CD150 among WT and Gata2R396Q/+ FL HSPCs at E12.5 (n = 11–18 embryos). K Number of CFU subtypes from E12.5 FL in WT or Gata2R396Q/+ embryos (n = 11–18 embryos). L Schematic for transplantation of E12.5 FL cells. M Peripheral blood CD45.2+ chimerism in recipients of E12.5 FL (n = 9–12 recipients). Peripheral blood output of recipients, showing CD45.2+ chimerism within myeloid and lymphoid lineages N or lineage output from CD45.2+ cells O (n = 9–12 recipients). ns not significant, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. E14.5, embryonic day 14.5; FL fetal liver, CFU colony-forming unit, LSK lineage-Sca-1+c-Kit+, LT-HSC long-term hematopoietic stem cell, ST-HSC short-term HSC, MPP2 multipotent progenitor 2, gMFI geometric mean fluorescence intensity, GEMM granulocyte/erythrocyte/monocyte/megakaryocyte, BFU-E burst-forming unit-erythroid, G/M/GM granulocyte/monocyte.
