Fig. 5: LSD1 and DOT1L co-occupy enhancers in MPN-BP cells.

A Tornado-plots visualizing the occupancy of DOT1L at LSD1 binding sites in HEL cells. The map was created based on regions with LSD1-ChIPseq peaks (MACS2) and sorted by LSD1 signal intensity. DOT1L-ChIPseq signals were mapped to these regions showing a large degree of co-occupancy. B Pie-chart showing the assignment of LSD1 and DOT1L co-occupied sites to different regions in the genome (ChIPseeker output). C Tornado-plots visualizing the deposition of H3K79me2 and H3K4me1 at LSD1 and DOT1L co-occupied regions. Map was sorted based on H3K79me2 signal intensities. D Tornado-plots visualizing the occupancy of DOT1L at LSD1 binding sites in primary MPN-BP patient samples. The map was created based on regions with LSD1-ChIPseq peaks (MACS2) and sorted by LSD1 signal intensity. Regions with signal enrichment in the corresponding input samples were deemed artefacts and removed. DOT1L-ChIPseq signals were mapped to these regions showing a large degree of co-occupancy. E Pie-chart showing the assignment of LSD1 and DOT1L co-occupied sites in MPN-BP samples to different regions in the genome (ChIPseeker output). F Genome-browser (IGV / Integrative Genome Viewer) tracks showing DOT1L, LSD1 and H3K4me1 co-occupancy at selected regulatory regions in HEL cells. G Tornado-plots visualizing LSD1-signal intensity at LSD1 + DOT1L co-occupied genomic sites in DOT1L-WT and DOT1L-ko HEL cells.