Table 1 sgRNA sequences and oligonucleotides.
sgRNAs: | ||
|---|---|---|
Name | Sequence 5’ → 3’ | Source |
sgMLL.intron 9 | U*U*C*CUAGGCUAGAACAUGUG + 80-mer Synthego modified EZ Scaffold | Synthego |
sgAF4.intron 3 | C*A*G*UGUGGCGUGUAUUACGU + 80-mer Synthego modified EZ Scaffold | Synthego |
Oligonucleotides: | ||
|---|---|---|
Name | Sequence 5’ → 3’ | Source |
MLL.ex9.sg.gDNA fw | CAAAAACCAAAAGAAAAGGTGAGGAGA | Eurofins |
MLL.ex10.gDNA rev | ATTGACCGGAGGTGGTTTTTCCTAAG | Eurofins |
AF4.I3.sg.1 gDNA fw | CTTATTTCTTCCTCAAAACAACCCTGTAAG | Eurofins |
AF4.I3.sg.1 gDNA rev | GGGGAAGGAATAGGGGGAAGAT | Eurofins |
MLL 8.3 | CCCAAAACCACTCCTAGTGAG | Eurofins |
MLL 13.5 | CAGGGTGATAGCTGTTTCGG | Eurofins |
AF4.3 | GTTGCAATGCAGCAGAAGCC | Eurofins |
AF4.5 | ACTGTCACTGTCCTCACTGTC | Eurofins |
