Fig. 4: ATAC-seq analysis identified chromatin accessibility status that may be associated with PRDM1 binding in human NK-cells.
From: Key regulatory roles of PRDM1 in human NK-cell differentiation and activation
A Overlapping peaks between ATAC-seq and PRDM1 ChIP-seq. The density plots show distribution of overlapping peak numbers in 1000 random shuffle permutations with Bedtools and the red lines denote the observed overlap numbers and their p-values. B ATAC-seq signals showing PRDM1 (top) and RUNX (bottom) footprints in NK-IL2-D6 and NK-F-D13. C Bar graph showing the number and percentage of PRDM1-bound sites with PRDM1 and/or RUNX motifs that were accessible or inaccessible. P-value indicates the significance of Chi-squared test of percentage between two groups. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Bar graph showing number and percentage D and KEGG pathway analysis E of PRDM1 footprint-containing genes that showed differential ATAC signal between NK-IL2-d6 or NK-F-D13 vs. freshly isolated CD56dim NK-cells. P value indicates significance in Chi-squared test of the proportion.
