Fig. 6: Proteomic analysis of PRDM1-associated proteins.
From: Key regulatory roles of PRDM1 in human NK-cell differentiation and activation
A Schematic overview of the RIME assay. B RIME assay identified transcription cofactors associated with PRDM1 in primary human NK-cells or in NKYS cells, which are grouped into corepressors, coactivator/transcription activators, and BAF complex components. The dataset from IgG control group was used to remove nonspecific background. C Schematic overview of the APEX2 proximity-based biotinylation assay. D PRDM1-associated cofactors identified by the APEX2 experiment. Proteins with more than two-fold increase of the normalized peptide count in APEX2 knock-in samples compared to Cas9 control were shown.
