Fig. 1: KMT2A::MLLT3+ CSF1R+ LMPPs have a higher clonogenic potential and are more promiscuous than KMT2A::MLLT3+ CSF1R- LMPPs. | Leukemia

Fig. 1: KMT2A::MLLT3+ CSF1R+ LMPPs have a higher clonogenic potential and are more promiscuous than KMT2A::MLLT3+ CSF1R- LMPPs.

From: CSF1R marks a subset of foetal haematopoietic multipotent progenitor cells with acute myeloid leukaemia propagation properties

Fig. 1

A Experimental layout. B Type 1 (T1), Type 2 (T2), and Type 3 (T3) colony output of CSF1R + /- KMT2A::MLLT3+ LMPPs in CFU-lymphoid assays (N = 4, from 2 experiments/litters); right panel showing representative pictures of Type 1 (T1), Type 2 (T2), Type 3 (T3) colonies. All pictures were acquired at 4X magnification. C Phenotype of CSF1R- and CSF1R+ colonies at the end of the CFU-lymphoid assay. Data shown as percent of B220+ lymphoid, CD11b+ myeloid cells in the CD45+ population (N = 3). D Representative flow cytometry plots showing the cell phenotype of CSF1R- and CSF1R+ colonies at the end of the CFU-lymphoid assay. E BLAST, CFU-GM, CFU-M, CFU-G colony output of CSF1R + /- KMT2A::MLLT3+ LMPPs in CFU-myeloid assay (N = 3, from 2 experiments/litters). F Phenotype of CSF1R- and CSF1R+ colonies at the end of the CFU-myeloid assay. Data shown as percent of B220+ lymphoid, CD11b+ myeloid and B220 + CD11b+ mixed-phenotype cells in the CD45+ population. G Representative flow cytometry plots showing the cell phenotype of CSF1R- and CSF1R+ colonies at the end of the CFU-myeloid assay (N = 3). Data are presented as mean with SD, and statistical analysis was calculated with 2-way ANOVA test: p < 0.01 (**) p < 0.001 (***), p < 0.0001 (****). CFU-GM (colony-forming unit-granulocytes/macrophages), CFU-M (colony-forming unit macrophages), CFU-G (colony-forming unit granulocytes).

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