Fig. 5: Synergistic effect of ^AlloCAR70-NKT cells with HMA in the treatment of CD70-positive AML using a THP1-FG xenograft mouse model.

A Experimental design to study the synergistic effect of ^AlloCAR70-NKT cells with Decitabine in vivo. A human CD70⁺ AML cell line THP1-FG was utilized. B BLI images showing the presence of THP1-FG tumor cells in experimental mice over time. C Quantification of (B) (n = 5). D Kaplan–Meier survival curves (n = 5). E ELISA analyses of human IFN-γ and TNF-α levels in serum collected from experimental mice on day 20. F FACS analyses of the percentage of tumor cells (identified as GFP⁺ cells) among total live cells in bone marrow collected from experimental mice at the study endpoint. G FACS detection of ^AlloCAR70-NKT cells in mouse tissues on day 35. H Quantification of (G) (n = 5). I FACS detection of CD69 expression, as well as Perforin and Granzyme B production by ^AlloCAR70-NKT cells in mouse bone marrow on day 35. J Quantification of (I) (n = 5). Representative of 3 experiments. Data are presented as the mean ± SEM. ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, by Student’s t test (H and J), one-way ANOVA (E and F), two-way ANOVA (C), or log rank (Mantel-Cox) test adjusted for multiple comparisons (D). i.v. intravenous, TBL total bioluminescence.