Fig. 1: Precision and accuracy in localization microscopy.
From: Subnanometer localization accuracy in widefield optical microscopy
a Schematic showing a linear target. b Schematic showing a logarithmic target. Green dots are localization data. Their scatter indicates statistical uncertainty at the subnanometer scale, which is not apparent on the linear target as systematic errors can be four orders of magnitude larger. This discrepancy requires a logarithmic target to illustrate both precision and accuracy. Calibration of the measurement system and correction of localization data ensures that precision is the limit of accuracy13
