Fig. 4: Axial calibration of mirror-enhanced dSTORM.
From: Sharpening emitter localization in front of a tuned mirror
a Color-coded map of the z-position of A647 fluorophores labeling a 15 µm microsphere imaged in the SSD configuration (upper panel) and the mirror-enhanced dSTORM configuration (lower panel). The z-position was calculated based on the microsphere radius (diffraction-limited image of the equatorial plane, inset upper panel) and the radial position ρ of the single localization events respective to the bead center (inset lower panel). b Axial dependence of the intensity (left) and the localization uncertainty (right) of single events localized in the experiment in the SSD (upper graphs, gray dots) and the mirror-enhanced dSTORM (lower graphs, blue dots) configurations. The solid lines mark the height-dependent average intensity and average localization uncertainty. c Comparison of the height-dependent intensity profile of the SSD (gray) and mirror-enhanced (blue) configurations with the simulated emission enhancement (solid light gray line) and excitation enhancement (dashed light gray line). The height regions in which the excitation enhancement drops below 1 are highlighted in light gray. Scale bars: 5 µm
