Fig. 1: MMF imaging system for minimally invasive deep-brain in vivo imaging achieves diffraction-limited spatial resolution. | Light: Science & Applications

Fig. 1: MMF imaging system for minimally invasive deep-brain in vivo imaging achieves diffraction-limited spatial resolution.

From: Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber

Fig. 1: MMF imaging system for minimally invasive deep-brain in vivo imaging achieves diffraction-limited spatial resolution.The alternative text for this image may have been generated using AI.

a Schematic of the experimental system. (i) The source unit distributes the laser light for fluorescence excitation and calibration. (ii) The portable imaging arm controls the light propagation through the MMF by wavefront shaping using an LC-SLM. (iii) The calibration unit is used for the acquisition of the transmission matrix prior to imaging. (iv) The sample unit holds tissue sections and head-fixed mice. DM dichroic mirror, MMF multimode fiber, MO microscope objective, PMT photomultiplier tube, SMF single-mode fiber. b Relationship between image resolution and instrument footprint for in vivo light-based imaging modalities (Supplementary Table S1). The MMF data point is shown in blue. c Picture of a mouse brain (bottom) with optical components for in vivo imaging (top): a 60× water immersion objective (left), a GRIN lens (middle), and the 50-μm-core MMF used in this study (right)

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