Fig. 2: Laser particles for comprehensive single-cell analyses.
From: Multiplexed laser particles for spatially resolved single-cell analysis
a Left: SEM of a silica-coated, III–V semiconductor microdisk laser particle (LP). Middle: Narrowband emission measured from the LP upon optical pumping with a 1060 nm laser emitting 3 ns pulses at 2 MHz and 20 pJ/pulse. Right: Use of different materials and fabrication of different microdisk diameters by nanolithography yields 400 LPs with single-mode emission from 1170 to 1580 nm. Reproduced from Ref 25. b Spectral barcoding by using combinations of different LPs per cell. c A human cell (HEK293) carrying three LPs. Magenta (LP), green (membrane), blue (nucleus). d Left: Fabrication of multiplet LPs. Right: An example of emission from a triplet LP, one of ~11 million barcodes possible assuming 400 spectral channels. e Proposed workflow for comprehensive single-cell analysis using LPs. Tissue-containing laser particles are imaged to capture tissue architecture and cellular behaviors. LP emission is measured before and after dissociation to track cell identity. Any downstream molecular analysis of single cells (e.g., transcriptomics and proteomics) can be correlated to their location and behavior in their native tissue
