Fig. 3: PV-1 simultaneously delivers up to three cell-impermeable organic fluorescent probes into live cells. | Light: Science & Applications

Fig. 3: PV-1 simultaneously delivers up to three cell-impermeable organic fluorescent probes into live cells.

From: Simple and efficient delivery of cell-impermeable organic fluorescent probes into live cells for live-cell superresolution imaging

Fig. 3: PV-1 simultaneously delivers up to three cell-impermeable organic fluorescent probes into live cells.

a Confocal microscopy images of live U-2 OS cells after a 1-h coincubation with PV-1 (4 μM), Tubulin-FITC (5 μM), and Hoechst-Alexa 647 (3 μM). Scale bar: 50 μm. b Confocal microscopy images of live U-2 OS cells wild-type (top) or expressing CLIP-Sec61β (bottom left) or SNAP-Sec61β (bottom right) after a 1-h coincubation with PV-1 (4 μM) and the two indicated probes (Hoechst-Alexa 647: 3 μM; the others: 5 μM). Scale bars: 10 μm. c A triple-color SIM image of live U-2 OS cells expressing SNAP-Sec61β after a 1-h coincubation with PV-1 (4 μM), Tubulin-Atto 565 (5 μM), SNAP-Atto 488 (5 μM), and Hoechst-Alexa 647 (3 μM). Scale bar: 2 μm. The labeled organelles are indicated in the panels

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