Fig. 3: Evaluation of the point spread function (PSF) of CLAM.
a (Left) CLAM images of the nanobeads (dispersed on a tilted cover slide) for the PSF measurement (volume of 90 × 40 × 25 μm3); (right) the projected 2D PSFs measured from two beads (beads 1 and 2). The scale bars are 2 μm. b Statistics (n = 13) of the measured resolutions along the three dimensions (represented in boxplots) within the volume of 90 × 40 × 25 μm3. c Implementation of the extended depth of field (DOF) based on the induced spherical aberration, which is implemented by placing a PDMS block between the sample (a tilted cover slide with dispersed nanobeads) and the detection objective. d Comparison of the DOFs between the CLAM images obtained with and without the PDMS block. The depth is color coded. The scale bars represent (top) 50 μm and (bottom) 20 μm. e Measured full-width-half-maximum (FWHM) (relative to the minimal value) of the PSF along the depth (z) in the cases (red) without and (blue) with the PDMS block. The solid curves are a quadratic fit. The DOF is extended by ~32% in the presence of spherical aberration. f Imaging of microparticles flowing in the micropipette with a volumetric frame rate of 13.2 vol/s (volume of 170 × 25 × 28 μm3).
