Fig. 1: Hardware implementation, algorithm pipeline, and resolution benchmarking of SR-FACT. | Light: Science & Applications

Fig. 1: Hardware implementation, algorithm pipeline, and resolution benchmarking of SR-FACT.

From: Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome

Fig. 1

a Schematic diagram of the SR-FACT setup. b Flow chart of the ODT reconstruction algorithm. c Flow chart of the SIM reconstruction algorithm. d Representative example of a COS-7 cell labeled with LifeAct-EGFP imaged with SR-FACT. Scale bar, 5 μm. e Average intensity profiles of RIs (orange) and fluorescence (green) along lines orthogonal to the actin filaments (with the same filament at 50 time points); a representative example is shown in (d). f Average full width at half maximum (FWHM) values of the profiles orthogonal to the actin filament labeled by the lines in (d) measured at 50 different time points. Center lines, medians; limits, 75 and 25%; whiskers, maximum and minimum.

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