Fig. 3: Elemental distribution imaged by scanning X-ray fluorescence nanoprobe in cultured primary neurons, a cellular model of AD. | Light: Science & Applications

Fig. 3: Elemental distribution imaged by scanning X-ray fluorescence nanoprobe in cultured primary neurons, a cellular model of AD.

From: Correlative optical photothermal infrared and X-ray fluorescence for chemical imaging of trace elements and relevant molecular structures directly in neurons

Fig. 3

a Elemental distribution imaged by scanning X-ray fluorescence nanoprobe in cultured primary neurons, a cellular model of AD. a Elemental distribution of Cl, Cu, and Fe in cultured APP-KO neurons incubated for 30 min with 5 × 10−6 M synthetic Aβ(1–42). Maps of Cl are used for neuronal identification. Pixel size is 500 nm; scale bar is 40 µm. b Averaged and normalized S-XRF sum-spectra of cultured mouse primary neurons. Blue spectrum corresponds to APP-KO treated with Aβ(1–42); black spectrum corresponds to untreated neurons. (N = 3 cells per APP-KO embryo, from a total of 3 embryos). c Average area of the Fe clusters calculated per cell, including soma and neurites. d Iron distribution in untreated APP-KO primary neurons. Scale bar is 20 µm

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