Fig. 3: OLID-SDOM imaging of subcellular organelles in live budding yeast cells. | Light: Science & Applications

Fig. 3: OLID-SDOM imaging of subcellular organelles in live budding yeast cells.

From: Polarization modulation with optical lock-in detection reveals universal fluorescence anisotropy of subcellular structures in live cells

Fig. 3

a Wide-field epifluorescence microscopy images of GFP labeled subcellular organelles, such as lipid particles, late Golgi, actin, spindle pole, peroxisome, ER to Golgi vesicle, Golgi complex, and endosome. Scale bar: 5 μm. The diagrams inset is the Gac of reconstructed OLID-SDOM images of the white boxed area. Scale bar: 2 μm. b Anisotropy distribution of different subcellular organelles indicates that subcellular organelles, which have a different function in cells, have specific OUF.

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