Fig. 4: Fluorescence imaging with P-CHNPs and in vitro anticancer effect of P-CHNPs. | Light: Science & Applications

Fig. 4: Fluorescence imaging with P-CHNPs and in vitro anticancer effect of P-CHNPs.

From: Light amplified oxidative stress in tumor microenvironment by carbonized hemin nanoparticles for boosting photodynamic anticancer therapy

Fig. 4

a Confocal fluorescence images of HEK293 and 4T1 cells treated with the P-CHNPs. [P-CHNPs] = 100 μg mL−1. Scale bar: 50 μm. b Optical images of Calcein-AM and PI co-stained 4T1 cells treated with nothing (Control), light irradiation (Light), P-CHNPs (PS), and both light irradiation and P-CHNPs (PDT). Light irradiation: 400–700 nm, 100 mW cm−2, 20 min. [P-CHNPs] = 200 μg mL−1. Calcein-AM (Ex: 488 nm; Em: 505–525 nm) and PI (Ex: 552 nm; Em: 605–625 nm), [Calcein-AM] = 2 μM, [PI] = 2 μM. Scale bar: 200 μm

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